22 February 2008 Cell-population tracking using quantum dots in flow cytometry
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Abstract
We have used flow-cytometry together with computational modeling of quantum dot portioning during cell division to identify population distributions of proliferating cells. The objective has been to develop a robust assay of integrated cellular fluorescence which reports the extent of cellular bifurcation within a complex population and potentially provides profiles of drug resistance, cell clonality and levels of aneuploidy in tumour cells. The implementation of a data analysis program based on genetic algorithms provides a complete description of the proliferation dynamics and gives values for the inter-mitotic time, the partitioning ratio of quantum dots between daughter cells and their associated deviations.
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Huw D. Summers, Huw D. Summers, Rachel J. Errington, Rachel J. Errington, Paul J. Smith, Paul J. Smith, Sally Chappell, Sally Chappell, Paul Rees, Paul Rees, Martyn R. Brown, Martyn R. Brown, James F. Leary, James F. Leary, } "Cell-population tracking using quantum dots in flow cytometry", Proc. SPIE 6865, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications V, 68650L (22 February 2008); doi: 10.1117/12.763602; https://doi.org/10.1117/12.763602
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