Specific in vivo labeling with GFP retroviruses, lentiviruses, and adenoviruses for imaging

Robert M. Hoffman; Hiroyuki Kishimoto; Toshiyoshi Fujiwara

doi:10.1117/12.773308

29 February 2008 Specific in vivo labeling with GFP retroviruses, lentiviruses, and adenoviruses for imaging

Robert M. Hoffman, Hiroyuki Kishimoto, Toshiyoshi Fujiwara

Author Affiliations +

Proceedings Volume 6868, Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes; 68680F (2008) https://doi.org/10.1117/12.773308
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Fluorescent proteins have revolutionized the field of imaging. Our laboratory pioneered in vivo imaging with fluorescent proteins. Fluorescent proteins have enabled imaging at the subcellular level in mice. We review here the use of different vectors carrying fluorescent proteins to selectively label normal and tumor tissue in vivo. We show that a GFP retrovirus and telomerase-driven GFP adenovirus can selectively label tumors in mice. We also show that a GFP lentivirus can selectively label the liver in mice. The practical application of these results are discussed.

Citation Download Citation

Robert M. Hoffman, Hiroyuki Kishimoto, and Toshiyoshi Fujiwara "Specific in vivo labeling with GFP retroviruses, lentiviruses, and adenoviruses for imaging", Proc. SPIE 6868, Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes, 68680F (29 February 2008); https://doi.org/10.1117/12.773308

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