23 February 2008 Nano-aquarium for dynamic observation of aquatic microorganisms fabricated by femtosecond laser direct writing of photostructurable glass
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Abstract
We demonstrate the fabrication of three-dimensional (3-D) hollow microstructures embedded in photostructurable glass by a femtosecond (fs) laser direct writing. Fs laser direct writing followed by annealing and successive wet etching in dilute hydrofluoric (HF) acid solution resulted in the rapid manufacturing of microchips with 3-D hollow microstructures for the dynamic observation of living microorganisms in fresh water. The embedded microchannel structure enables us to analyze the continuous motion of Euglena gracilis. A microchamber with a movable microneedle demonstrates its ability for the elucidation of the information transmission process in Pleurosira laevis. Such microchips, referred to as nano-aquariums realize the efficient and highly functional observation of microorganisms.
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Y. Hanada, K. Sugioka, H. Kawano, I. Ishikawa, A. Miyawaki, K. Midorikawa, "Nano-aquarium for dynamic observation of aquatic microorganisms fabricated by femtosecond laser direct writing of photostructurable glass", Proc. SPIE 6880, Laser-based Micro- and Nanopackaging and Assembly II, 68800E (23 February 2008); doi: 10.1117/12.762391; https://doi.org/10.1117/12.762391
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