12 February 2008 A nanofluidic system for massively parallel PCR
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Massively parallel nanofluidic systems are lab-on-a-chip devices where solution phase biochemical and biological analyses are implemented in high density arrays of nanoliter holes micro-machined in a thin platen. Polymer coatings make the interior surfaces of the holes hydrophilic and the exterior surface of the platen hydrophobic for precise and accurate self-metered loading of liquids into each hole without cross-contamination. We have created a "nanoplate" based on this concept, equivalent in performance to standard microtiter plates, having 3072 thirty-three nanoliter holes in a stainless steel platen the dimensions of a microscope slide. We report on the performance of this device for PCR-based single nucleotide polymorphism (SNP) genotyping or quantitative measurement of gene expression by real-time PCR in applications ranging from plant and animal diagnostics, agricultural genetics and human disease research.
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Colin Brenan, Colin Brenan, Tom Morrison, Tom Morrison, Douglas Roberts, Douglas Roberts, James Hurley, James Hurley, } "A nanofluidic system for massively parallel PCR", Proc. SPIE 6886, Microfluidics, BioMEMS, and Medical Microsystems VI, 688609 (12 February 2008); doi: 10.1117/12.772243; https://doi.org/10.1117/12.772243

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