12 February 2008 A nanofluidic system for massively parallel PCR
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Abstract
Massively parallel nanofluidic systems are lab-on-a-chip devices where solution phase biochemical and biological analyses are implemented in high density arrays of nanoliter holes micro-machined in a thin platen. Polymer coatings make the interior surfaces of the holes hydrophilic and the exterior surface of the platen hydrophobic for precise and accurate self-metered loading of liquids into each hole without cross-contamination. We have created a "nanoplate" based on this concept, equivalent in performance to standard microtiter plates, having 3072 thirty-three nanoliter holes in a stainless steel platen the dimensions of a microscope slide. We report on the performance of this device for PCR-based single nucleotide polymorphism (SNP) genotyping or quantitative measurement of gene expression by real-time PCR in applications ranging from plant and animal diagnostics, agricultural genetics and human disease research.
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Colin Brenan, Colin Brenan, Tom Morrison, Tom Morrison, Douglas Roberts, Douglas Roberts, James Hurley, James Hurley, "A nanofluidic system for massively parallel PCR", Proc. SPIE 6886, Microfluidics, BioMEMS, and Medical Microsystems VI, 688609 (12 February 2008); doi: 10.1117/12.772243; https://doi.org/10.1117/12.772243
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