One of the key measures of response to treatment for patients in multicenter clinical trials is the lung density measured in Hounsfield Units (HU) from Computer Tomography (CT) scans. The purpose of this work is to determine the dependence of CT attenuation values on scanner type by using in vivo measurements made from homogeneous anatomic areas. In vivo measurements were made in areas within the trachea, aorta, fat and muscle regions of CT scans obtained from subjects scanned as part of a multicenter treatment trial. Scans were selected so that exams from all four major manufacturers were included in the study. For each anatomic region of interest, the mean and standard deviation values were computed to investigate attenuation dependence on scanners. For example, trachea mean (standard deviation) measurements for exams from GE, Siemens, Philips and Toshiba scanners were -986 HU(±15), - 993 HU(±9), -988HU(±8), -1046(±10) respectively. Inter-scanner variability was observed for each scanner showing significant differences (all p-values <0.005). Previous work in examining attenuation dependence on scanners has been performed using anthropomorphic phantoms. The novelty of this work is the use of in vivo measurements from homogeneous regions in order to examine scanner effects on CT attenuation values. Our results show that CT attenuation values for the anatomic regions vary between scanners and hence, dependence of CT attenuation values on scanners is observed.