20 March 2008 Whole mouse cryo-imaging
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Abstract
The Case cryo-imaging system is a section and image system which allows one to acquire micron-scale, information rich, whole mouse color bright field and molecular fluorescence images of an entire mouse. Cryo-imaging is used in a variety of applications, including mouse and embryo anatomical phenotyping, drug delivery, imaging agents, metastastic cancer, stem cells, and very high resolution vascular imaging, among many. Cryo-imaging fills the gap between whole animal in vivo imaging and histology, allowing one to image a mouse along the continuum from the mouse -> organ -> tissue structure -> cell -> sub-cellular domains. In this overview, we describe the technology and a variety of exciting applications. Enhancements to the system now enable tiled acquisition of high resolution images to cover an entire mouse. High resolution fluorescence imaging, aided by a novel subtraction processing algorithm to remove sub-surface fluorescence, makes it possible to detect fluorescently-labeled single cells. Multi-modality experiments in Magnetic Resonance Imaging and Cryo-imaging of a whole mouse demonstrate superior resolution of cryo-images and efficiency of registration techniques. The 3D results demonstrate the novel true-color volume visualization tools we have developed and the inherent advantage of cryo-imaging in providing unlimited depth of field and spatial resolution. The recent results continue to demonstrate the value cryo-imaging provides in the field of small animal imaging research.
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David Wilson, David Wilson, Debashish Roy, Debashish Roy, Grant Steyer, Grant Steyer, Madhusudhana Gargesha, Madhusudhana Gargesha, Meredith Stone, Meredith Stone, Eliot McKinley, Eliot McKinley, } "Whole mouse cryo-imaging", Proc. SPIE 6916, Medical Imaging 2008: Physiology, Function, and Structure from Medical Images, 69161I (20 March 2008); doi: 10.1117/12.772840; https://doi.org/10.1117/12.772840
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