Bioluminescent bioreporter assays for targeted detection of chemical and biological agents

Steven Ripp; Pat Jegier; Courtney Johnson; Scott Moser; Syed Islam; Gary Sayler

doi:10.1117/12.777274

15 April 2008 Bioluminescent bioreporter assays for targeted detection of chemical and biological agents

Steven Ripp, Pat Jegier, Courtney Johnson, Scott Moser, Syed Islam, Gary Sayler

Proceedings Volume 6945, Optics and Photonics in Global Homeland Security IV; 69450M (2008) https://doi.org/10.1117/12.777274
Event: SPIE Defense and Security Symposium, 2008, Orlando, Florida, United States

Abstract

Bioluminescent bioreporters carrying the bacterial lux gene cassette have been well established for the sensing and monitoring of select chemical agents. Their ability to generate target specific visible light signals with no requirement for extraneous additions of substrate or other hands-on manipulations affords a real-time, repetitive assaying technique that is remarkable in its simplicity and accuracy. Although the predominant application of lux-based bioluminescent bioreporters has been towards chemical compound detection, novel genetic engineering schemes are yielding a variety of new bioreporter systems that extend the lux sensing mechanism beyond mere analyte discrimination. For example, the unique specificity of bacteriophage (bacterial viruses) has been exploited in lux bioluminescent assays for specific identification of foodborne bacterial pathogens such as Escherichia coli O157:H7. With the concurrent ability to interface bioluminescent bioreporter assays onto integrated circuit microluminometers (BBICs; bioluminescent bioreporter integrated circuits), the potential exists for the development of sentinel microchips that can function as environmental monitors for multiplexed recognition of chemical and biological agents in air, food, and water. The size and portability of BBIC biosensors may ultimately provide a deployable, interactive network sensing technology adaptable towards chem/bio defense.

Citation Download Citation

Steven Ripp, Pat Jegier, Courtney Johnson, Scott Moser, Syed Islam, and Gary Sayler "Bioluminescent bioreporter assays for targeted detection of chemical and biological agents", Proc. SPIE 6945, Optics and Photonics in Global Homeland Security IV, 69450M (15 April 2008); https://doi.org/10.1117/12.777274

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