Refractive index measurement of biological particles in visible region

D. Limsui; A. K. Carr; M. E Thomas; N. T Boggs; R. I Joseph

doi:10.1117/12.777648

21 April 2008 Refractive index measurement of biological particles in visible region

D. Limsui; A. K. Carr; M. E Thomas; N. T Boggs; R. I Joseph;

Author Affiliations +

Proceedings Volume 6954, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing IX; 69540X (2008) https://doi.org/10.1117/12.777648
Event: SPIE Defense and Security Symposium, 2008, Orlando, Florida, United States

Abstract

Optical cross-sections of biological warfare simulants, killed agents, and live agents are needed to assess the standoff detection performance of active lidar and passive FTIR systems. To aid in this investigation, Johns Hopkins University Applied Physics Laboratory (JHU/APL) has developed a technique to determine the index of refraction of biological materials in the visible region using a combination of transmission measurements and anomalous diffraction theory (ADT). The spectral measurements using a dual beam grating spectrometer provide a basis for calculating the optical cross section of suspended particles. ADT is then used to convert the cross section result into index of refraction. A summary of this procedure is described along with the results for silica microspheres and Bacillus globijii (BG). A comparison of these results to published data is also presented.

Citation Download Citation

D. Limsui, A. K. Carr, M. E Thomas, N. T Boggs, R. I Joseph, "Refractive index measurement of biological particles in visible region", Proc. SPIE 6954, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing IX, 69540X (21 April 2008); doi: 10.1117/12.777648; https://doi.org/10.1117/12.777648

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