30 April 2008 Detection of nucleic acid hybridization via oxide-gated carbon nanotube field-effect transistors
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Abstract
A label-free DNA hybridization detector using carbon nanotube transistor arrays is developed. The sensors are comprised of a network of carbon nanotubes covered by thin oxide layer, which serve as efficient charge transducers for biomolecules in solution. Probe DNA sequences are immobilized on the gate oxide, and the conductance is measured before and after exposure and hybridization with a target DNA. Complementary binding results in a net charge doubling at the oxide surface which induces a positive shift in the threshold voltage and concomitant increase in the current at fixed bias. The method does not involve chemical functionalization of the carbon nanotubes and is compatible with protocols in conventional DNA microarrays. Most importantly, the technique does not require reporter molecules or tagging labels, which greatly simplifies the operation and reduces the cost. We have shown a measurable response to hybridization with target concentration of ~1 nM. The implementation, theory of operation, device fabrication and solutions to pertinent engineering issues to build practical system are discussed.
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Konrad H. Aschenbach, Herman Pandana, Jookyung Lee, Javed Khan, Michael Fuhrer, Dan Lenski, R. D. Gomez, "Detection of nucleic acid hybridization via oxide-gated carbon nanotube field-effect transistors", Proc. SPIE 6959, Micro (MEMS) and Nanotechnologies for Space, Defense, and Security II, 69590W (30 April 2008); doi: 10.1117/12.778531; https://doi.org/10.1117/12.778531
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