You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither SPIE nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the SPIE website.
30 April 2008Detection of nucleic acid hybridization via oxide-gated carbon nanotube field-effect transistors
A label-free DNA hybridization detector using carbon nanotube transistor arrays is developed. The sensors are
comprised of a network of carbon nanotubes covered by thin oxide layer, which serve as efficient charge transducers for
biomolecules in solution. Probe DNA sequences are immobilized on the gate oxide, and the conductance is measured
before and after exposure and hybridization with a target DNA. Complementary binding results in a net charge doubling
at the oxide surface which induces a positive shift in the threshold voltage and concomitant increase in the current at
fixed bias. The method does not involve chemical functionalization of the carbon nanotubes and is compatible with
protocols in conventional DNA microarrays. Most importantly, the technique does not require reporter molecules or
tagging labels, which greatly simplifies the operation and reduces the cost. We have shown a measurable response to
hybridization with target concentration of ~1 nM. The implementation, theory of operation, device fabrication and
solutions to pertinent engineering issues to build practical system are discussed.
The alert did not successfully save. Please try again later.
Konrad H. Aschenbach, Herman Pandana, Jookyung Lee, Javed Khan, Michael Fuhrer, Dan Lenski, R. D. Gomez, "Detection of nucleic acid hybridization via oxide-gated carbon nanotube field-effect transistors," Proc. SPIE 6959, Micro (MEMS) and Nanotechnologies for Space, Defense, and Security II, 69590W (30 April 2008); https://doi.org/10.1117/12.778531