18 April 2008 Rapid screening and species identification of E. coli, Listeria, and Salmonella by SERS technique
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Abstract
Techniques for routine and rapid screening of the presence of foodborne bacteria are needed, and this study reports the feasibility of citrate-reduced silver colloidal SERS for identifying E. coli, Listeria, and Salmonella. Relative standard deviation (RSD) of SERS spectra from silver colloidal suspensions and ratios of P-O SERS peaks from small molecule (K3PO4) were used to assess the reproducibility, stability, and binding effectiveness of citrate-reduced silver colloids over batch and storage process. The results suggested the reproducibility of silver colloids over batch process and also stability and consistent binding effectiveness over 60-day storage period. Notably, although silver colloidal nanoparticles were stable for at least 90 days, their binding effectiveness began to decrease slightly after 60-day storage, with a binding reduction of about 12% at 90th day. Colloidal silver SERS, as demonstrated here, could be an important alternative technique in the rapid and simultaneous screening of the presence of three most outbreak bacteria due to the exclusive biomarkers, label-free and easy sampling attribute.
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Yongliang Liu, Yongliang Liu, Kuanglin Chao, Kuanglin Chao, Moon S. Kim, Moon S. Kim, Xiangwu Nou, Xiangwu Nou, } "Rapid screening and species identification of E. coli, Listeria, and Salmonella by SERS technique", Proc. SPIE 6983, Defense and Security 2008: Special Sessions on Food Safety, Visual Analytics, Resource Restricted Embedded and Sensor Networks, and 3D Imaging and Display, 698309 (18 April 2008); doi: 10.1117/12.786881; https://doi.org/10.1117/12.786881
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