2 May 2008 Identification of active fluorescence stained bacteria by Raman spectroscopy
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Abstract
Microorganisms can be found everywhere e.g. in food both as useful ingredients or harmful contaminations causing food spoilage. Therefore, a fast and easy to handle analysis method is needed to detect bacteria in different kinds of samples like meat, juice or air to decide if the sample is contaminated by harmful microorganisms. Conventional identification methods in microbiology require always cultivation and therefore are time consuming. In this contribution we present an analysis approach to identify fluorescence stained bacteria on strain level by means of Raman spectroscopy. The stained bacteria are highlighted and can be localized easier against a complex sample environment e.g. in food. The use of Raman spectroscopy in combination with chemometrical methods allows the identification of single bacteria within minutes.
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Mario Krause, Mario Krause, Beatrice Beyer, Beatrice Beyer, Christian Pietsch, Christian Pietsch, Benno Radt, Benno Radt, Michaela Harz, Michaela Harz, Petra Rösch, Petra Rösch, Jürgen Popp, Jürgen Popp, } "Identification of active fluorescence stained bacteria by Raman spectroscopy", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910E (2 May 2008); doi: 10.1117/12.781321; https://doi.org/10.1117/12.781321
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