How to measure slow diffusion in yeast cell membranes

Jonas Ries; Christian Klose; Christiane Walch-Solimena; Petra Schwille

doi:10.1117/12.787043

2 May 2008 How to measure slow diffusion in yeast cell membranes

Jonas Ries, Christian Klose, Christiane Walch-Solimena, Petra Schwille

Author Affiliations +

Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 69910W (2008) https://doi.org/10.1117/12.787043
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

Here we present two complementary methods for accurate diffusion measurements in yeast cell membranes. Fluorescence spreading after photobleaching analyzes the blurring of an initially sharp border between bleached and unbleached parts of the membrane. Two-focus scanning fluorescence correlation spectroscopy requires only a low concentration of labeled fluorophores and allows for very long measurement times due to correction for instabilities necessary to probe the slow diffusion in yeast plasma membranes. We apply these techniques to study the dynamics of different transmembrane proteins in the plasma membrane of the yeast Saccharomyces cerevisiae. The differences in the diffusion coefficients support the idea of co-existing membrane microdomains in the yeast plasma membrane.

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Jonas Ries, Jonas Ries, Christian Klose, Christian Klose, Christiane Walch-Solimena, Christiane Walch-Solimena, Petra Schwille, Petra Schwille, } "How to measure slow diffusion in yeast cell membranes", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910W (2 May 2008); doi: 10.1117/12.787043; https://doi.org/10.1117/12.787043

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