2 May 2008 Determination of the integral refractive index of cells in suspension by digital holographic phase contrast microscopy
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Abstract
A digital holographic microscopy method for the determination of the integral refractive index of living cells in suspension is presented. Therefore, digital holographic phase contrast images of trypsinized cells in suspension are recorded. Afterwards, the cell radius and the integral cellular refractive index are determined by fitting of a two dimensional model. The applied algorithm requires only information about the refractive index of the suspension medium and the scale of the microscopic imaging system. The fitting algorithm is characterized on simulated phase data and digital holographic phase contrast images of beads. Results obtained from living human pancreas tumor cells demonstrate the applicability of the method for cell characterization.
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Sebastian Kosmeier, Sebastian Kosmeier, Björn Kemper, Björn Kemper, Patrik Langehanenberg, Patrik Langehanenberg, Ilona Bredebusch, Ilona Bredebusch, Jürgen Schnekenburger, Jürgen Schnekenburger, Andreas Bauwens, Andreas Bauwens, Gert von Bally, Gert von Bally, } "Determination of the integral refractive index of cells in suspension by digital holographic phase contrast microscopy", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 699110 (2 May 2008); doi: 10.1117/12.781125; https://doi.org/10.1117/12.781125
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