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8 May 2008 Optical detection of PNA/DNA hybridization in resonant porous silicon-based devices
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Abstract
The development of label-free optical biosensors could have a great impact on life sciences as well as on screening techniques for medical and environmental applications. Peptide nucleic acid (PNA) is a nucleic acid analog in which the sugar phosphate backbone of natural nucleic acid has been replaced by a synthetic peptide backbone, resulting in an achiral and uncharged mimic. Due to the uncharged nature of PNA, PNA-DNA duplexes show a better thermal stability respect the DNA-DNA equivalents. In this work, we used an optical biosensor, based on the porous silicon (PSi) nanotechnology, to detect PNA-DNA interactions. PSi optical sensors are based on changes of reflectivity spectrum when they are exposed to the target analytes. The porous silicon surface was chemically modified to covalently link the PNA which acts as a very specific probe for its ligand (cDNA).
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Lucia Rotiroti, Paolo Arcari, Annalisa Lamberti, Carmen Sanges, Edoardo De Tommasi, Ilaria Rea, Ivo Rendina, and Luca De Stefano "Optical detection of PNA/DNA hybridization in resonant porous silicon-based devices", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 699120 (8 May 2008); doi: 10.1117/12.781147; https://doi.org/10.1117/12.781147
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