Improved antimicrobial therapy with cationic tetra- and octa-substituted phthalocyanines

I. Angelov; V. Mantareva; V. Kussovski; D. Woehrle; E. Borisova; L. Avramov

doi:10.1117/12.822519

19 December 2008 Improved antimicrobial therapy with cationic tetra- and octa-substituted phthalocyanines

I. Angelov, V. Mantareva, V. Kussovski, D. Woehrle, E. Borisova, L. Avramov

Proceedings Volume 7027, 15th International School on Quantum Electronics: Laser Physics and Applications; 702717 (2008) https://doi.org/10.1117/12.822519
Event: 15th International School on Quantum Electronics: Laser Physics and Applications, 2008, Bourgas, Bulgaria

Abstract

Photodynamic therapy (PDT) today is an innovative and not yet widespread light-drug initiated treatment that is based on the photoactive compound irradiated with proper light to produce oxygen species that are toxic to the pathogenic biological objects- bacteria, viruses, tumor cells. The obstacles that limited the efficacy of PDT concern to the selectivity and multi-drug resistance prolong time for cellular release and side effects of skin photosensitivity for commercial porphyrin originated photosensitizers (PS). Now there are very intensive investigations for introducing in practice a new, with a least side effects PSs for PDT. The usefulness of the more extended macromolecules structured with proper substituents refers not only to the improved optical properties like far-red and with intensive absorption and emission capacity, but mainly to the ability for selective delivery and adhesion to the target cells, such as bacteria or other pathogens. The present study focuses on the charge effect of photodynamic agent on the uptake capacity toward gram-negative bacteria cells and their further photoinactivation. The multi-drug resistant microorganism Aeromanas hydrophilla, which is causing diseases to fishes and humans, is treated. The new octa-cationic phthalocyanines are designed to compare PDT efficacy to the efficacy of tetra-substituted derivatives with the same functional peripheral substituents. The higher cellular accumulation to the bacteria cells as a result of the high number of positive charges of photosensitizer, leading to the better adhesion to the cellular membranes and improved photoinactivation of bacteria causing superficial and intraorgan infections. These results set a base of a rationale design of covalently octa-substituted phthalocyanines with positive charge for a successful treatment of microorganisms.

Citation Download Citation

I. Angelov, V. Mantareva, V. Kussovski, D. Woehrle, E. Borisova, and L. Avramov "Improved antimicrobial therapy with cationic tetra- and octa-substituted phthalocyanines", Proc. SPIE 7027, 15th International School on Quantum Electronics: Laser Physics and Applications, 702717 (19 December 2008); https://doi.org/10.1117/12.822519

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