Hardware-based anti-Brownian electrokinetic trap (ABEL trap) for single molecules: control loop simulations and application to ATP binding stoichiometry in multi-subunit enzymes

Yan Jiang; Quan Wang; Adam E. Cohen; Nick Douglas; Judith Frydman; W. E. Moerner

doi:10.1117/12.798093

29 August 2008 Hardware-based anti-Brownian electrokinetic trap (ABEL trap) for single molecules: control loop simulations and application to ATP binding stoichiometry in multi-subunit enzymes

Yan Jiang, Quan Wang, Adam E. Cohen, Nick Douglas, Judith Frydman, W. E. Moerner

Author Affiliations +

Proceedings Volume 7038, Optical Trapping and Optical Micromanipulation V; 703807 (2008) https://doi.org/10.1117/12.798093
Event: NanoScience + Engineering, 2008, San Diego, California, United States

Abstract

The hardware-based Anti-Brownian ELectrokinetic trap (ABEL trap) features a feedback latency as short as 25 μs, suitable for trapping single protein molecules in aqueous solution. The performance of the feedback control loop is analyzed to extract estimates of the position variance for various controller designs. Preliminary data are presented in which the trap is applied to the problem of determining the distribution of numbers of ATP bound for single chaperonin multi-subunit enzymes.

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Yan Jiang, Quan Wang, Adam E. Cohen, Nick Douglas, Judith Frydman, and W. E. Moerner "Hardware-based anti-Brownian electrokinetic trap (ABEL trap) for single molecules: control loop simulations and application to ATP binding stoichiometry in multi-subunit enzymes", Proc. SPIE 7038, Optical Trapping and Optical Micromanipulation V, 703807 (29 August 2008); https://doi.org/10.1117/12.798093

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