Study of optically trapped living Trypanosoma cruzi/Trypanosoma rangeli - Rhodnius prolixus interactions by real time confocal images using CdSe quantum dots

A. A. de Thomaz; D. B. Almeida; W. M. Faustino; G. J. Jacob; A. Fontes; L. C. Barbosa; C. L. Cesar; C. V. Stahl; J. R. Santos-Mallet; S. A. O. Gomes; D. Feder

doi:10.1117/12.795370

29 August 2008 Study of optically trapped living Trypanosoma cruzi/Trypanosoma rangeli - Rhodnius prolixus interactions by real time confocal images using CdSe quantum dots

A. A. de Thomaz, D. B. Almeida, W. M. Faustino, G. J. Jacob, A. Fontes, L. C. Barbosa, C. L. Cesar, C. V. Stahl, J. R. Santos-Mallet, S. A. O. Gomes, D. Feder

Author Affiliations +

Proceedings Volume 7038, Optical Trapping and Optical Micromanipulation V; 703810 (2008) https://doi.org/10.1117/12.795370
Event: NanoScience + Engineering, 2008, San Diego, California, United States

Abstract

One of the fundamental goals in biology is to understand the interplay between biomolecules of different cells. This happen, for example, in the first moments of the infection of a vector by a parasite that results in the adherence to the cell walls. To observe this kind of event we used an integrated Optical Tweezers and Confocal Microscopy tool. This tool allow us to use the Optical Tweezers to trigger the adhesion of the Trypanosoma cruzi and Trypanosoma rangeli parasite to the intestine wall cells and salivary gland of the Rhodnius prolixus vector and to, subsequently observe the sequence of events by confocal fluorescence microscopy under optical forces stresses. We kept the microorganism and vector cells alive using CdSe quantum dot staining. Besides the fact that Quantum Dots are bright vital fluorescent markers, the absence of photobleaching allow us to follow the events in time for an extended period. By zooming to the region of interested we have been able to acquire confocal images at the 2 to 3 frames per second rate.

Citation Download Citation

A. A. de Thomaz, D. B. Almeida, W. M. Faustino, G. J. Jacob, A. Fontes, L. C. Barbosa, C. L. Cesar, C. V. Stahl, J. R. Santos-Mallet, S. A. O. Gomes, and D. Feder "Study of optically trapped living Trypanosoma cruzi/Trypanosoma rangeli - Rhodnius prolixus interactions by real time confocal images using CdSe quantum dots", Proc. SPIE 7038, Optical Trapping and Optical Micromanipulation V, 703810 (29 August 2008); https://doi.org/10.1117/12.795370

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