In vivo blood lactic acid monitoring using microdialysis and surface-enhanced Raman spectroscopy

Po-Hsiang Hsu; Tung-Hu Tsai; Huihua Kenny Chiang

doi:10.1117/12.794798

9 September 2008 In vivo blood lactic acid monitoring using microdialysis and surface-enhanced Raman spectroscopy

Po-Hsiang Hsu, Tung-Hu Tsai, Huihua Kenny Chiang

Proceedings Volume 7040, Nanobiosystems: Processing, Characterization, and Applications; 70400R (2008) https://doi.org/10.1117/12.794798
Event: NanoScience + Engineering, 2008, San Diego, California, United States

Abstract

Blood lactic acid concentration is an important indicator for physiological functions. To develop a rapid and sensitive measurement technique for monitoring blood lactic acid may provide a useful tool in clinical diagnosis. We proposed to develop a microdialysis surface-enhanced Raman spectroscopy (microdialysis-SERS) approach to filter/reduce interference from other large metabolites in blood and enhance the detection sensitivity for blood lactic acid. In this study, a microdialysis probe was constructed using 13 kDa cut-off dialysis membrane. The dialysate was mixed with 50 nm Ag colloidal nanoparticles automatically in a micro-fluid chamber for SERS detection under blood microdialysis of Sprague-Dawley rat. The linear range of SERS-lactic acid measurement is 10^-5~3x10^-4 M with R² value of 0.99. The optimal mixing flow rate of nanoparticles is 18 μl/min under microdialysis at constant flow rate (2 μl/min). Real time lactic acid monitoring in vivo also has been demonstrated using microdialysis-SERS system.

Citation Download Citation

Po-Hsiang Hsu, Tung-Hu Tsai, and Huihua Kenny Chiang "In vivo blood lactic acid monitoring using microdialysis and surface-enhanced Raman spectroscopy", Proc. SPIE 7040, Nanobiosystems: Processing, Characterization, and Applications, 70400R (9 September 2008); https://doi.org/10.1117/12.794798

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