9 September 2008 In vivo blood lactic acid monitoring using microdialysis and surface-enhanced Raman spectroscopy
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Blood lactic acid concentration is an important indicator for physiological functions. To develop a rapid and sensitive measurement technique for monitoring blood lactic acid may provide a useful tool in clinical diagnosis. We proposed to develop a microdialysis surface-enhanced Raman spectroscopy (microdialysis-SERS) approach to filter/reduce interference from other large metabolites in blood and enhance the detection sensitivity for blood lactic acid. In this study, a microdialysis probe was constructed using 13 kDa cut-off dialysis membrane. The dialysate was mixed with 50 nm Ag colloidal nanoparticles automatically in a micro-fluid chamber for SERS detection under blood microdialysis of Sprague-Dawley rat. The linear range of SERS-lactic acid measurement is 10-5~3x10-4 M with R2 value of 0.99. The optimal mixing flow rate of nanoparticles is 18 μl/min under microdialysis at constant flow rate (2 μl/min). Real time lactic acid monitoring in vivo also has been demonstrated using microdialysis-SERS system.
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Po-Hsiang Hsu, Po-Hsiang Hsu, Tung-Hu Tsai, Tung-Hu Tsai, Huihua Kenny Chiang, Huihua Kenny Chiang, } "In vivo blood lactic acid monitoring using microdialysis and surface-enhanced Raman spectroscopy", Proc. SPIE 7040, Nanobiosystems: Processing, Characterization, and Applications, 70400R (9 September 2008); doi: 10.1117/12.794798; https://doi.org/10.1117/12.794798

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