10 February 2009 Ultra-high-speed bionanoscope for cell and microbe imaging
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Proceedings Volume 7126, 28th International Congress on High-Speed Imaging and Photonics; 712605 (2009) https://doi.org/10.1117/12.822466
Event: 28th International Congress on High-Speed Imaging and Photonics, 2008, Canberra, Australia
Abstract
We are developing an ultra-high-sensitivity and ultra-high-speed imaging system for bioscience, mainly for imaging of microbes with visible light and cells with fluorescence emission. Scarcity of photons is the most serious problem in applications of high-speed imaging to the scientific field. To overcome the problem, the system integrates new technologies consisting of (1) an ultra-high-speed video camera with sub-ten-photon sensitivity with the frame rate of more than 1 mega frames per second, (2) a microscope with highly efficient use of light applicable to various unstained and fluorescence cell observations, and (3) very powerful long-pulse-strobe Xenon lights and lasers for microscopes. Various auxiliary technologies to support utilization of the system are also being developed. One example of them is an efficient video trigger system, which detects a weak signal of a sudden change in a frame under ultra-high-speed imaging by canceling high-frequency fluctuation of illumination light. This paper outlines the system with its preliminary evaluation results.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
T. Goji Etoh, T. Goji Etoh, Cuong Vo Le, Cuong Vo Le, Hiroyuki Kawano, Hiroyuki Kawano, Ikuko Ishikawa, Ikuko Ishikawa, Atshushi Miyawaki, Atshushi Miyawaki, Vu T. S. Dao, Vu T. S. Dao, Hoang Dung Nguyen, Hoang Dung Nguyen, Sayoko Yokoi, Sayoko Yokoi, Shigeru Yoshida, Shigeru Yoshida, Hitoshi Nakano, Hitoshi Nakano, Kohsei Takehara, Kohsei Takehara, Yoshiharu Saito, Yoshiharu Saito, } "Ultra-high-speed bionanoscope for cell and microbe imaging", Proc. SPIE 7126, 28th International Congress on High-Speed Imaging and Photonics, 712605 (10 February 2009); doi: 10.1117/12.822466; https://doi.org/10.1117/12.822466
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