Digital pinhole filter used for detection of confocal microscopy images

Xiangdong Huang; Na Liu; Junning Cui

doi:10.1117/12.821249

12 January 2009 Digital pinhole filter used for detection of confocal microscopy images

Xiangdong Huang, Na Liu, Junning Cui

Proceedings Volume 7133, Fifth International Symposium on Instrumentation Science and Technology; 71333N (2009) https://doi.org/10.1117/12.821249
Event: International Symposium on Instrumentation Science and Technology, 2008, Shenyang, China

Abstract

When conventional physical pinholes are used, the alignment of pinholes is always a problem. An iterative Wiener deconvolution filter is used to reject out-of-focus light, a sub-pixel centre location method is used to locate the centre of the pinhole, and the dimensions of the pinhole are optimized using the confocal microscopic theory. A filter with digital pinhole is thus developed and compared with filter with conventional physical pinhole through comparative tests. Experimental results indicate that the alignment of pinholes can be made easy, a filter with digital pinhole can be used to take place of a filter with conventional physical pinhole, and it can also reduce the pixel crosstalk resulting from multipoint illumination.

Citation Download Citation

Xiangdong Huang, Na Liu, and Junning Cui "Digital pinhole filter used for detection of confocal microscopy images", Proc. SPIE 7133, Fifth International Symposium on Instrumentation Science and Technology, 71333N (12 January 2009); https://doi.org/10.1117/12.821249

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