12 January 2009 Digital pinhole filter used for detection of confocal microscopy images
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Proceedings Volume 7133, Fifth International Symposium on Instrumentation Science and Technology; 71333N (2009) https://doi.org/10.1117/12.821249
Event: International Symposium on Instrumentation Science and Technology, 2008, Shenyang, China
Abstract
When conventional physical pinholes are used, the alignment of pinholes is always a problem. An iterative Wiener deconvolution filter is used to reject out-of-focus light, a sub-pixel centre location method is used to locate the centre of the pinhole, and the dimensions of the pinhole are optimized using the confocal microscopic theory. A filter with digital pinhole is thus developed and compared with filter with conventional physical pinhole through comparative tests. Experimental results indicate that the alignment of pinholes can be made easy, a filter with digital pinhole can be used to take place of a filter with conventional physical pinhole, and it can also reduce the pixel crosstalk resulting from multipoint illumination.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Xiangdong Huang, Na Liu, Junning Cui, "Digital pinhole filter used for detection of confocal microscopy images", Proc. SPIE 7133, Fifth International Symposium on Instrumentation Science and Technology, 71333N (12 January 2009); doi: 10.1117/12.821249; https://doi.org/10.1117/12.821249
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