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18 November 2008 Digital holographic phase contrast microscopy: a new technology in biophotonics
Gert von Bally
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Proceedings Volume 7138, Photonics, Devices, and Systems IV; 71380M (2008) https://doi.org/10.1117/12.817986
Event: Photonics, Devices, and Systems IV, 2008, Prague, Czech Republic
Abstract
Progress in gene- and biotechnology has opened a new application domain of optics - i.e. biophotonics. In this field, optical methods are developed and applied to the functional analysis of tissues and cells as well as to cell manipulation. Growth processes, e.g. in the developing stage of tumours, reveal themselves through changes in their elasticity properties and by micro movements. Holographic-interferometric methods have proven their capability to analyse local elasticity distribution and micro movements. In combination with microscopic optics, these "microinterferometric" methods provide a new basic technology - i.e. Digital Holographic Phase Contrast Microscope - for the analysis, manipulation and utilisation of living systems down to the nanoscale. Modular digital holographic microscopy allows maker-free, quantitative, contactless, fast, full field, high resolving (< 5 nm axially) imaging and analysis of living cells and can be combined simultaneously with most of the modern microscopic technologies. In this way, diagnostics standards for cell differentiation and manipulation as well as for cellular tissue engineering and other life sciences domains can be extended.
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Gert von Bally "Digital holographic phase contrast microscopy: a new technology in biophotonics", Proc. SPIE 7138, Photonics, Devices, and Systems IV, 71380M (18 November 2008); https://doi.org/10.1117/12.817986
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