Rapid development of micromanipulation and experimental techniques increases the need for easy-to-handle tools, which
are suitable for routine operations. Our new concept represents a very suitable platform for incorporating such new experimental
techniques into standard laboratory microscopy equipment. Implementing of microscopy methods such as optical
trapping, cutting, fluorescence excitation, photopolymerization and most spectroscopic techniques is very expensive and
time consuming. In most cases, microscope parts and body must be adapted to insert additional lasers, detectors etc. We
overcome these difficulties by inserting source of radiation directly between microscope body and microscope objective.
Our concept allows using arbitrary source of radiation (laser diodes, solid-state lasers, gas lasers). Laser diode source can
be incorporated inside the adapter. Optical fiber is used to deliver other sources of radiation. Light microscopy adapter
(LMA) can be easily mounted on most types of microscopes including special types as confocal microscope.
We demonstrate ability of LMA for stable optical trapping of particle in the range of hundreds of nanometers to tens
of micrometers, bleaching (FRAP, FLIP) and microdissection experiments. Mechanical and optical construction of LMA
addresses spatial constrains of commercially available microscopes, mainly the fact that optical parts of microscopes are
optimized for visible spectrum of light. Our solution preserves all microscope properties unaffected and it is possible
modern microscopy techniques to many types of microscopes.