Space-resolved fluorescence spectroscopic measurements with an optical fiber probe

Enbang Li; Hialin Qiu

doi:10.1117/12.812009

2 February 2009 Space-resolved fluorescence spectroscopic measurements with an optical fiber probe

Enbang Li, Hialin Qiu

Proceedings Volume 7157, 2008 International Conference on Optical Instruments and Technology: Advanced Sensor Technologies and Applications; 71570G (2009) https://doi.org/10.1117/12.812009
Event: International Conference of Optical Instrument and Technology, 2008, Beijing, China

Abstract

By monitoring of the emitted signal from a sample while varying the excitation wavelength, emission wavelength or both of them, fluorescence spectroscopy has become a powerful diagnostic technology. Fluorescence spectrometers can be used to measure and record the fluorescence spectra of a given sample, and have been successfully applied in different areas including biology, biochemistry, chemistry, medicine, environmental science, material science, food industry, and pharmaceutical industry. In order to increase the flexibility and applicability of conventional fluorescence spectrometers, we design an optic fiber probe for conducting the UV/Vis excitation light to a sample under study, and for collecting the fluorescence produced by the sample. Different excitation/emission fiber bundle arrangements have been fabricated and their performances have been evaluated and compared. Fiber adaptors which can be used for different commercial fluorescence spectrometers are also developed. In order to achieve space-resolved fluorescence spectroscopic measurements, we connect the fiber probe to a microscope which is mounted on a 3D traverse stage. Experiments and measurement results using the space-resolved fiber optic fluorescence spectrometer are presented in this paper.

Citation Download Citation

Enbang Li and Hialin Qiu "Space-resolved fluorescence spectroscopic measurements with an optical fiber probe", Proc. SPIE 7157, 2008 International Conference on Optical Instruments and Technology: Advanced Sensor Technologies and Applications, 71570G (2 February 2009); https://doi.org/10.1117/12.812009

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