2 February 2009 Micro amperometric immunosensor for the detection of salmonella typhimurium
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Abstract
In this paper, a micro amperometric immunosensor based on Micro-Electro-Mechanical Systems technology for the detection of Salmonella typhimurium (S. typhimurium) was constructed by immobilizing a polyclonal antibody (the bio-molecular recognition element) onto the surface of polypyrrole(PPy) /staphylococcal protein A(SPA) modified Pt electrode. Pyrrole doped with SPA was co-electropolymerized onto the working electrode surface by cyclic voltammetry in 10 minutes for orientation-controlled immobilization of salmonella capture antibodies. S. typhimurium with the concentration of 102cfu/ml could be detected by this immunosensor with a controllable and convenient manipulation to effectively modify the sensing surface more rapidly with less consumption of reagent (10µL), which showed the good property of the sensor. It is potential to develop a micro biosensor that can be used for convenient, accurate, cost-effective and real-time sensing of pathogens in food products.
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Jizhou Sun, Jizhou Sun, Shanhong Xia, Shanhong Xia, Chao Bian, Chao Bian, Lan Qu, Lan Qu, } "Micro amperometric immunosensor for the detection of salmonella typhimurium", Proc. SPIE 7159, 2008 International Conference on Optical Instruments and Technology: MEMS/NEMS Technology and Applications, 71590A (2 February 2009); doi: 10.1117/12.807108; https://doi.org/10.1117/12.807108
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