Paper
19 February 2009 Fluorescence spectroscopy for endogenous porphyrins in human facial skin
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Abstract
The activity of certain bacteria in skin is known to correlate to the presence of porphyrins. In particular the presence of coproporphyrin produced by P.acnes inside plugged pores has been correlated to acne vulgaris. Another porphyrin encountered in skin is protoporphyrin IX, which is produced by the body in the pathway for production of heme. In the present work, a fluorescence spectroscopy system was developed to measure the characteristic spectrum and quantify the two types of porphyrins commonly present in human facial skin. The system is comprised of a Xe lamp both for fluorescence excitation and broadband light source for diffuse reflectance measurements. A computer-controlled filter wheel enables acquisition of sequential spectra, first excited by blue light at 405 nm then followed by the broadband light source, at the same location. The diffuse reflectance spectrum was used to correct the fluorescence spectrum due to the presence of skin chromophores, such as blood and melanin. The resulting fluorescence spectra were employed for the quantification of porphyrin concentration in a population of healthy subjects. The results show great variability on the concentration of these porphyrins and further studies are being conducted to correlate them with skin conditions such as inflammation and acne vulgaris.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
I. Seo, S. H. Tseng, G. O. Cula, P. R. Bargo, and N. Kollias "Fluorescence spectroscopy for endogenous porphyrins in human facial skin", Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 716103 (19 February 2009); https://doi.org/10.1117/12.811913
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Cited by 17 scholarly publications.
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KEYWORDS
Luminescence

Skin

Fluorescence spectroscopy

Diffuse reflectance spectroscopy

Optical filters

Bacteria

Blood

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