17 February 2009 Laser speckle imaging of rat retinal blood flow with hybrid temporal and spatial analysis method
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Proceedings Volume 7163, Ophthalmic Technologies XIX; 716304 (2009) https://doi.org/10.1117/12.809906
Event: SPIE BiOS, 2009, San Jose, California, United States
Noninvasive monitoring of blood flow in retinal circulation will reveal the progression and treatment of ocular disorders, such as diabetic retinopathy, age-related macular degeneration and glaucoma. A non-invasive and direct BF measurement technique with high spatial-temporal resolution is needed for retinal imaging. Laser speckle imaging (LSI) is such a method. Currently, there are two analysis methods for LSI: spatial statistics LSI (SS-LSI) and temporal statistical LSI (TS-LSI). Comparing these two analysis methods, SS-LSI has higher signal to noise ratio (SNR) and TSLSI is less susceptible to artifacts from stationary speckle. We proposed a hybrid temporal and spatial analysis method (HTS-LSI) to measure the retinal blood flow. Gas challenge experiment was performed and images were analyzed by HTS-LSI. Results showed that HTS-LSI can not only remove the stationary speckle but also increase the SNR. Under 100% O2, retinal BF decreased by 20-30%. This was consistent with the results observed with laser Doppler technique. As retinal blood flow is a critical physiological parameter and its perturbation has been implicated in the early stages of many retinal diseases, HTS-LSI will be an efficient method in early detection of retina diseases.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Haiying Cheng, Haiying Cheng, Yumei Yan, Yumei Yan, Timothy Q. Duong, Timothy Q. Duong, } "Laser speckle imaging of rat retinal blood flow with hybrid temporal and spatial analysis method", Proc. SPIE 7163, Ophthalmic Technologies XIX, 716304 (17 February 2009); doi: 10.1117/12.809906; https://doi.org/10.1117/12.809906

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