Characterization of the vitreous body of the human eye using a cyanine dye as a spectral and fluorescent probe

Ina G. Panova; Alexander S. Tatikolov

doi:10.1117/12.808400

23 February 2009 Characterization of the vitreous body of the human eye using a cyanine dye as a spectral and fluorescent probe

Ina G. Panova, Alexander S. Tatikolov

Author Affiliations +

Proceedings Volume 7163, Ophthalmic Technologies XIX; 71631O (2009) https://doi.org/10.1117/12.808400
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We used one of cyanine dyes as a spectral and fluorescent probe in the study of the composition of the extracellular matrix of the human eye (its vitreous body). Owing to the unique ability of the dye to bind to collagens and human serum albumin, we revealed the simultaneous presence of both types of biomacromolecules in the vitreous body. The formation of the dye complex with human serum albumin leads to appearance of a long-wavelength absorption band (~612 nm) and a steep rise of fluorescence, whereas in the presence of collagens the dye forms J-aggregates with a longer-wavelength absorption band (640-660 nm) and moderate fluorescence. In this work we studied the composition of the human fetus vitreous body and its dynamics from 9 to 31 gestation weeks. On the basis of the data obtained by this method, we may assume that albumin, being a carrier protein, probably provides the vitreous body and surrounding tissues with necessary growth factors, hormones, lipids, vitamins, and some other biomolecules. The data show that the dye is promising not only for study of albumin functions in eye development, but also for characterization of some eye diseases and for analysis of other extracellular media.

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Ina G. Panova and Alexander S. Tatikolov "Characterization of the vitreous body of the human eye using a cyanine dye as a spectral and fluorescent probe", Proc. SPIE 7163, Ophthalmic Technologies XIX, 71631O (23 February 2009); https://doi.org/10.1117/12.808400

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