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18 February 2009 The role of reactive oxygen species in PDT efficacy
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Abstract
While the first reactive oxygen species (ROS) formed during photodynamic therapy (PDT) is singlet molecular oxygen (1O2), other ROS are formed downstream including superoxide anion radical (•CO2 -), hydrogen peroxide (H2O2) and hydroxyl radical (•OH). In this study, we examined the role of H2O2 in the phototoxic response to PDT in murine leukemia L388 cells. Inhibition of catalase activity, a major pathway to H2O2 detoxification, led to enhanced apoptosis and cell death. Addition of exogenous catalase offered protection from phototoxicity as did chelation of Fe+2, a co-factor in •OH production from H2O2. These results indicate the H2O2 formed during PDT plays a role in PDT efficacy.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Michael Price, Nakaiya Okan-Mensah, Ann Marie Santiago, and David Kessel "The role of reactive oxygen species in PDT efficacy", Proc. SPIE 7164, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII, 716402 (18 February 2009); https://doi.org/10.1117/12.810231
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