Imaging of a targeted PDT drug with fluorescence tomography

Dan Muffoletto; Anurag Gupta; Zhiqiang Xu; Chris Mahrer; Gretchen Bauer; Scott Galas; Ravindra K. Pandey; Ulas Sunar

doi:10.1117/12.809526

18 February 2009 Imaging of a targeted PDT drug with fluorescence tomography

Dan Muffoletto, Anurag Gupta, Zhiqiang Xu, Chris Mahrer, Gretchen Bauer, Scott Galas, Ravindra K. Pandey, Ulas Sunar

Proceedings Volume 7164, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII; 716409 (2009) https://doi.org/10.1117/12.809526
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We constructed a whole-body fluorescence tomography instrument to monitor novel bifunctional phototherapeutic drugs (e.g., HPPH-Cyanine dye conjugate) in small animals. The instrument allows dense source and detector sampling with a fast galvo scanner and a CCD detector for improved resolution and sensitivity (Patwardhan et al., 2005). Here we report tissue phantom measurements to evaluate the imaging performance with a newly constructed tomography instrument. Phantom measurements showed that strong fluorescence generated by HPPH-Cyanine dye (HPPH-CD), having high fluorescence quantum yield and long wavelength fluorescence emission, allowed deep tissue imaging. We also report in vivo fluorescence measurements of the conjugate in Nude mice bearing A549 human non-small cell lung carcinoma (NSCLC) tumors at 24 hr post injection to evaluate tumor detection ability of the conjugate. Our results indicate that the HPPH-CD shows preferential uptake in tumors compared to surrounding normal tissue at 24 hr post injection. This study demonstrates a potential use of HPPH-CD in detection (fluorescence imaging) and treatment (PDT) of deeply seated tumors.

Citation Download Citation

Dan Muffoletto, Anurag Gupta, Zhiqiang Xu, Chris Mahrer, Gretchen Bauer, Scott Galas, Ravindra K. Pandey, and Ulas Sunar "Imaging of a targeted PDT drug with fluorescence tomography", Proc. SPIE 7164, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII, 716409 (18 February 2009); https://doi.org/10.1117/12.809526

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