18 February 2009 Effects of a polarized light source (λ400-2000nm) on H.Ep.2 and L929 cell lines: a spectroscopic in vitro study
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Abstract
Aim: The aim of the present investigation was to compare, using the MTT Assay, the effects of a polarized light system (λ400-2000nm, 40mW, φ5cm, 9.6J/cm2) on cellular cultures of human laryngeal carcinoma (H.Ep.2) and in a fibroblast lineage (L929-CLLINCTC clone 929). Summary Background Data: Recently there has been increased interest in the propagation of polarized light in randomly scattering media, such as biological tissues, because their high potential of applications, particularly in biomedical area. Materials and Methods: The illuminations were performed at the following times: T0 (24 hours after handling the cells) and T48 (equivalent to 48 hours after the first illumination). The cellular viability was assessed using MTT essay at the following times T0, T6, T12, T24, T48, T72. The results were analyzed using the Graphpad Prism® software. Results: The results showed that time had influenced on the cellular viability of L929 on both control (P=0.0014) and illuminated cultures (P=0.0035). Significant difference between control (P=0.0001) and illuminated H.Ep.2 cells (P=0.0001) was observed. There was a significant difference between the two used types of cells illuminated when compared to their controls: H.Ep.2 (P=0.0001) and L929 (P=0.0002). Conclusion: The use of polarized light on H.Ep.2 and L929 cells resulted on photobiological effects that need further investigation of the mechanisms involved as this is the first study using this methodology.
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Juliana Santos De Carvalho Monteiro, Katia Vale Monteiro de Moura, Cibelle Barbosa Lopes, Cristina Pacheco Soares, Antonio Luiz Barbosa Pinheiro, "Effects of a polarized light source (λ400-2000nm) on H.Ep.2 and L929 cell lines: a spectroscopic in vitro study", Proc. SPIE 7165, Mechanisms for Low-Light Therapy IV, 71650A (18 February 2009); doi: 10.1117/12.807942; https://doi.org/10.1117/12.807942
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