24 February 2009 Toward photostable multiplex analyte detection on a single mode planar optical waveguide
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Abstract
We have developed a waveguide-based optical biosensor for the sensitive and specific detection of biomarkers associated with disease. Our technology combines the superior optical properties of single-mode planar waveguides, the robust nature of functionalized self-assembled monolayer sensing films and the specificity of fluorescence sandwich immunoassays to detect biomarkers in complex biological samples such as serum, urine and sputum. We have previously reported the adaptation of our technology to the detection of biomarkers associated with breast cancer and anthrax. However, these approaches primarily used phospholipid bilayers as the functional film and organic dyes (ex: AlexaFluors) as the fluorescence reporter. Organic dyes are easily photodegraded and are not amenable to multiplexing because of their narrow Stokes' shift. Here we have developed strategies for conjugation of the detector antibodies with quantum dots for use in a multiplex detection platform. We have previously evaluated dihydroxylipoic acid quantum dots for the detection of a breast cancer biomarker. In this manuscript, we investigate the detection of the Bacillus anthracis protective antigen using antibodies conjugated with polymer-coated quantum dots. Kinetics of binding on the waveguide-based biosensor is reported. We compare the sensitivity of quantum dot labeled antibodies to those labeled with AlexaFluor and demonstrate the photostability of the former in our assay platform. In addition, we compare sulfydryl labeling of the antibody in the hinge region to that of nonspecific amine labeling. This is but the first step in developing a multiplex assay for such biomarkers on our waveguide platform.
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Harshini Mukundan, Harshini Mukundan, Hongzhi Xie, Hongzhi Xie, Aaron Anderson, Aaron Anderson, W. Kevin Grace, W. Kevin Grace, Jennifer S. Martinez, Jennifer S. Martinez, Basil Swanson, Basil Swanson, "Toward photostable multiplex analyte detection on a single mode planar optical waveguide", Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 71670A (24 February 2009); doi: 10.1117/12.809530; https://doi.org/10.1117/12.809530
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