23 February 2009 A sandwich assay for procalcitonin detection for POCT applications
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Abstract
A plastic biochip was developed for the detection of procalcitonin (PCT) and consists of a polymethylmethacrylate (PMMA) chip shaped in order to achieve several flow microchannels. A sandwich assay using a new antibody pairs is implemented with the capture antibody immobilized on the PMMA surface and the target antibody labelled with a fluorophore. A laser diode excites the fluorescent sensing layer. Thanks to the anisotropy of the fluorescence the emitted light travels along the thickness of the plastic material. The fluorescence coming out from the chip is collected by 1 mm plastic optical fibre and detected with a spectrum analyser.
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Francesco Baldini, Luca Bolzoni, Ambra Giannetti, Melanie Kess, Petra M. Kraemer, Elisabeth Kremmer, Giampiero Porro, Folco Senesi, Cosimo Trono, "A sandwich assay for procalcitonin detection for POCT applications", Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 716905 (23 February 2009); doi: 10.1117/12.810265; https://doi.org/10.1117/12.810265
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