23 February 2009 Tissue imaging of myocardial infarct regions by a slit-scanning Raman microscope
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Estimating the distribution of myocardial fibrosis after myocardial infarct is important for appropriate therapeutic planning. Here, we applied a Raman confocal microscope equipped with slit scanner for molecular tissue imaging of rat infarcted hearts. Raman spectra of the cytoplasm of cardiomyocytes included the resonance Raman bands at 751, 1130 and 1582 cm-1 arising mainly from reduced b- and c- type cytochromes. Raman spectra of fibrotic tissues at the borderzone of old myocardial infarct were highly consistent with that of collagen type I. Based on these findings, we successfully obtained Raman tissue images of a cardiomyocyte and surrounding collagen at the cellular level.
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Mitsugu Ogawa, Mitsugu Ogawa, Yoshinori Harada, Yoshinori Harada, Yoshihisa Yamaoka, Yoshihisa Yamaoka, Katsumasa Fujita, Katsumasa Fujita, Tetsuro Takamatsu, Tetsuro Takamatsu, "Tissue imaging of myocardial infarct regions by a slit-scanning Raman microscope", Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 71690H (23 February 2009); doi: 10.1117/12.808079; https://doi.org/10.1117/12.808079

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