In vivo fluorescence hyperspectral imaging of oral neoplasia

Darren Roblyer; Cristina Kurachi; Ann M. Gillenwater; Rebecca Richards-Kortum

doi:10.1117/12.807226

23 February 2009 In vivo fluorescence hyperspectral imaging of oral neoplasia

Darren Roblyer, Cristina Kurachi, Ann M. Gillenwater, Rebecca Richards-Kortum

Proceedings Volume 7169, Advanced Biomedical and Clinical Diagnostic Systems VII; 71690J (2009) https://doi.org/10.1117/12.807226
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

A hyperspectral imaging system using a liquid-crystal tunable filter (LCTF) was constructed for the purpose of in vivo optical imaging of oral neoplasia. The system operates in fluorescence mode and has the dual capability of capturing high quality widefield images and detecting fluorescence emission spectra from arbitrary locations within the captured field of view (FOV). The system was calibrated and evaluated for spectral resolution and accuracy. In vivo hyperspectral images were obtained from two normal volunteers and two patients with confirmed oral malignancy. Normal volunteer measurements revealed differences in intensity and lineshape of spectra between different anatomic locations, but intensity and lineshape were similar between different measurement sites from the same anatomic location. Measurements from normal and neoplastic areas of two patients with previously confirmed oral neoplasia showed differences in intensity, lineshape, and location of peak intensity. We have demonstrated that this system can provide both high quality widefield images, and spectral information at chosen locations within the field of view.

Citation Download Citation

Darren Roblyer, Cristina Kurachi, Ann M. Gillenwater, and Rebecca Richards-Kortum "In vivo fluorescence hyperspectral imaging of oral neoplasia", Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 71690J (23 February 2009); https://doi.org/10.1117/12.807226

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