One- and two-photon time-resolved fluorescence of visible and near-infrared dyes in scattering media

R. Esposito; C. Altucci; R. Velotta; G. M. Gaeta; M. Lepore

doi:10.1117/12.810006

23 February 2009 One- and two-photon time-resolved fluorescence of visible and near-infrared dyes in scattering media

R. Esposito, C. Altucci, R. Velotta, G. M. Gaeta, M. Lepore

Proceedings Volume 7169, Advanced Biomedical and Clinical Diagnostic Systems VII; 71691J (2009) https://doi.org/10.1117/12.810006
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Visible and near-infrared dyes are largely used in diagnostics and sensing. For this reason, it is very important to study their time-resolved fluorescence in presence or in absence of proper scattering medium in order to simulate the optical characteristics of biological tissues. Moreover, if one- or two-photon excitation processes are available also visible dyes will be employed taking advantages from using exciting sources in the diagnostic window (red and near IR) of the electromagnetic spectrum, where the photons are rarely absorbed and more often scattered. Visible and near IR fluorescent samples (Indocyanine Green and Rhodamine 6G) in absence and in presence of scattering agents (different Intralipid concentrations) and one- and two- photon time-resolved experiments have been performed. As expected, the presence of scattering agents modified time-resolved spectra and the related lifetime components. The experimental results have been used to preliminarly test different theoretical approaches describing the propagation of fluorescence signals in scattering media.

Citation Download Citation

R. Esposito, C. Altucci, R. Velotta, G. M. Gaeta, and M. Lepore "One- and two-photon time-resolved fluorescence of visible and near-infrared dyes in scattering media", Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 71691J (23 February 2009); https://doi.org/10.1117/12.810006

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