Bioluminescence tomography using spectral techniques

Hamid Dehghani; Scott C. Davis; Brian W. Pogue

doi:10.1117/12.807738

20 February 2009 Bioluminescence tomography using spectral techniques

Hamid Dehghani, Scott C. Davis, Brian W. Pogue

Proceedings Volume 7171, Multimodal Biomedical Imaging IV; 717104 (2009) https://doi.org/10.1117/12.807738
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Spectrally-resolved BioLuminescence optical Tomography (BLT) aims to reconstruct tomographic images of Luciferase activity within a volume using spectrally resolved emission data from internal bioluminescence sources. Uniqueness as associated with non-spectrally resolved intensity-based BioLuminescence Tomography is an established drawback and it is also demonstrated that without the use of a non-negative constraint inverse algorithm the solution is inaccurate. Reconstructed images of bioluminescence are presented showing that the location of the internal bioluminescence source can be obtained with 0.5 mm accuracy. The concept of the reciprocity approach for image reconstruction is outlined providing a dramatic improvement in computational time without loss to image accuracy.

Citation Download Citation

Hamid Dehghani, Scott C. Davis, and Brian W. Pogue "Bioluminescence tomography using spectral techniques", Proc. SPIE 7171, Multimodal Biomedical Imaging IV, 717104 (20 February 2009); https://doi.org/10.1117/12.807738

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