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20 February 2009 Bioluminescence tomography using spectral techniques
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Proceedings Volume 7171, Multimodal Biomedical Imaging IV; 717104 (2009)
Event: SPIE BiOS, 2009, San Jose, California, United States
Spectrally-resolved BioLuminescence optical Tomography (BLT) aims to reconstruct tomographic images of Luciferase activity within a volume using spectrally resolved emission data from internal bioluminescence sources. Uniqueness as associated with non-spectrally resolved intensity-based BioLuminescence Tomography is an established drawback and it is also demonstrated that without the use of a non-negative constraint inverse algorithm the solution is inaccurate. Reconstructed images of bioluminescence are presented showing that the location of the internal bioluminescence source can be obtained with 0.5 mm accuracy. The concept of the reciprocity approach for image reconstruction is outlined providing a dramatic improvement in computational time without loss to image accuracy.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Hamid Dehghani, Scott C. Davis, and Brian W. Pogue "Bioluminescence tomography using spectral techniques", Proc. SPIE 7171, Multimodal Biomedical Imaging IV, 717104 (20 February 2009);

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