Imaging protoporphyrin IX fluorescence with a time-domain FMT/microCT system

Frederic Leblond; Dax Kepshire; Julia A. O'Hara; Hamid Dehghani; Subha Srinivasan; N. Mincu; M. Hutchins; M. Khayat; B. W. Pogue

doi:10.1117/12.809267

20 February 2009 Imaging protoporphyrin IX fluorescence with a time-domain FMT/microCT system

Frederic Leblond, Dax Kepshire, Julia A. O'Hara, Hamid Dehghani, Subha Srinivasan, N. Mincu, M. Hutchins, M. Khayat, B. W. Pogue

Author Affiliations +

Proceedings Volume 7171, Multimodal Biomedical Imaging IV; 717106 (2009) https://doi.org/10.1117/12.809267
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Fluorescence molecular tomography (FMT) has the potential to become a powerful quantitative research tool for pre-clinical applications such as evaluating the efficacy of experimental drugs. In this paper, we show how a time-domain FMT/microCT instrument can in principle be used to monitor volumetric fluorescence intensity over time for low fluorophore concentration levels. The experimental results we present relate to Protoporphyrin IX which has a quantum efficiency as much as two orders of magnitude lower compared to more conventional extrinsic dyes used for molecular imaging (e.g., Alexa Fluor dyes, Cyanine dyes). Our results highlight the high sensitivity of the single photon counting technology on which the optical system we have built is based. In conjunction with this system we have developed a diffuse optical fluorescence reconstruction technique that is robust and shown here to perform adequately even in cases when the contribution of noise to the data is important. Related to this, we show that the regularization scheme we have developed is reliable even for low fluorophore concentration values and that no adjustment of the regularization parameter needs to be made for different levels of noise. This generic reconstruction approach insures that images reconstructed from data sets acquired at different times and for different fluorescence levels can be compared on an equal footing.

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Frederic Leblond, Dax Kepshire, Julia A. O'Hara, Hamid Dehghani, Subha Srinivasan, N. Mincu, M. Hutchins, M. Khayat, and B. W. Pogue "Imaging protoporphyrin IX fluorescence with a time-domain FMT/microCT system", Proc. SPIE 7171, Multimodal Biomedical Imaging IV, 717106 (20 February 2009); https://doi.org/10.1117/12.809267

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