Paper
20 February 2009 Breast cancer targeting novel microRNA-nanoparticles for imaging
Arutselvan Natarajan, Senthil K. Venugopal, Sally J. DeNardo M.D., Mark A. Zern M.D.
Author Affiliations +
Proceedings Volume 7171, Multimodal Biomedical Imaging IV; 71710N (2009) https://doi.org/10.1117/12.812186
Event: SPIE BiOS, 2009, San Jose, California, United States
Abstract
MicroRNAs (miRNAs) are one of the most prevalent small (~22 nucleotide) regulatory RNA classes in animals. These miRNAs constitute nearly one percent of genes in the human genome, making miRNA genes one of the more abundant types of regulatory molecules. MiRNAs have been shown to play important roles in cell development, apoptosis, and other fundamental biological processes. MiRNAs exert their influence through complementary base-pairing with specific target mRNAs, leading to degradation or translational repression of the targeted mRNA. We have identified and tested a novel microRNA (miR-491) and demonstrated increased apoptosis in hepatocellular carcinoma cells (HepG2) and in human breast cancer cells (HBT3477) in vitro. We prepared a novel cancer targeting assembly of gold nanoparticles (GNP) with Quantum dots, miR-491, and MAb-ChL6 coupled through streptavidin/biotin for effective transfection, and to induce apoptosis in specific cancer cells for imaging and targeted therapy. The targeting and apoptosis inducing ability was tested by confocal and electron microscopy. The MAb-GNP-miR491-Qdot construct effectively transfected into the HBT3477 cells and induced apoptosis the confirmation of these results would suggest a new class of molecules for the imaging and therapy of breast cancer.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Arutselvan Natarajan, Senthil K. Venugopal, Sally J. DeNardo M.D., and Mark A. Zern M.D. "Breast cancer targeting novel microRNA-nanoparticles for imaging", Proc. SPIE 7171, Multimodal Biomedical Imaging IV, 71710N (20 February 2009); https://doi.org/10.1117/12.812186
Lens.org Logo
CITATIONS
Cited by 4 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Cell death

Breast cancer

Gold

Cancer

Particles

Nanoparticles

Molecules

Back to Top