Two-photon in vivo flow cytometry using a fiber probe

Yu-Chung Chang; Jing Yong Ye; Thommey P. Thomas; Zhengyi Cao; Alina Kotlyar; Eric R. Tkaczyk; James R. Baker Jr.; Theodore B. Norris

doi:10.1117/12.808436

20 February 2009 Two-photon in vivo flow cytometry using a fiber probe

Yu-Chung Chang, Jing Yong Ye, Thommey P. Thomas, Zhengyi Cao, Alina Kotlyar, Eric R. Tkaczyk, James R. Baker Jr., Theodore B. Norris

Author Affiliations +

Proceedings Volume 7173, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications IX; 71730I (2009) https://doi.org/10.1117/12.808436
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We have demonstrated the use of a double-clad fiber probe to conduct two-photon excited flow cytometry in vitro and in vivo. We conducted two-channel detection to measure fluorescence at two distinct wavelengths simultaneously. Because the scattering and absorption problems from whole blood were circumvented by the fiber probe, the detected signal strength from the cells were found to be similar in PBS and in whole blood. We achieved the same detection efficiency of the membrane-binding lipophilic dye DiD labeled cells in PBS and in whole blood. High detection efficiency of green fluorescent protein (GFP)-expressing cells in whole blood was demonstrated. DiD-labeled untransfected and GFP-transfected cells were injected into live mice and the circulation dynamics of the externally injected cells were monitored. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed in whole blood.

Citation Download Citation

Yu-Chung Chang, Jing Yong Ye, Thommey P. Thomas, Zhengyi Cao, Alina Kotlyar, Eric R. Tkaczyk, James R. Baker Jr., and Theodore B. Norris "Two-photon in vivo flow cytometry using a fiber probe", Proc. SPIE 7173, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications IX, 71730I (20 February 2009); https://doi.org/10.1117/12.808436

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