23 February 2009 Raman spectroscopy and rotating orthogonal polarization imaging for non-destructive tracking of collagen deposition in tissue engineered skin and tendon
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Abstract
To ensure the sustainability of tissue engineered products there is a need to consider the engineering and manufacturing issues related to them particularly for the purposes of process optimization and product quality assurance. This work describes the application of Raman spectroscopy for in process monitoring of a skin substitute and rotating orthogonal polarization imaging to track collagen alignment in a tissue engineered tendon. The skin substitute studied is produced from culturing fibroblasts in a fibrin matrix. Throughout the production process the fibroblasts secrete extracellular matrix and in doing so deposit collagen in the matrix. Key to optimization of the skin substitute production process is development of strategies to track the collagen and fibrin content. The work presented here discusses the feasibility of Raman spectroscopy to resolve fibrin and collagen components in the skin substitutes. Collagen alignment is also important in the engineering of many tissues, in particular tendons. Thus, this work will also investigate the ability of rotating orthogonal polarization imaging to track collagen alignment in a tissue engineered tendon.
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Melissa L. Mather, Steve P. Morgan, David E. Morris, Qun Zhu, Jasmin Kee, Alina Zoladek, John A. Crowe, Ioan Notingher, David J. Williams, Penny A. Johnson, "Raman spectroscopy and rotating orthogonal polarization imaging for non-destructive tracking of collagen deposition in tissue engineered skin and tendon", Proc. SPIE 7179, Optics in Tissue Engineering and Regenerative Medicine III, 71790A (23 February 2009); doi: 10.1117/12.812221; https://doi.org/10.1117/12.812221
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