Application of laser to measurement of cyclic contractile movement of cultured myotubes

Shigehiro Hashimoto; Hiroyuki Tonami; Eiji Yamada; Shuichi Mochizuki; Jun Takase; Mieko Ohsuga

doi:10.1117/12.805234

12 February 2009 Application of laser to measurement of cyclic contractile movement of cultured myotubes

Shigehiro Hashimoto, Hiroyuki Tonami, Eiji Yamada, Shuichi Mochizuki, Jun Takase, Mieko Ohsuga

Proceedings Volume 7179, Optics in Tissue Engineering and Regenerative Medicine III; 71790D (2009) https://doi.org/10.1117/12.805234
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Laser technique has been applied to measure cyclic contractile movement of cultured myotubes in vitro. The designed measurement system includes light source (helium neon, 632.8 nm wave length), charge-coupled devise cameras and detectors. Cyclic contraction of myotubes cultured from C2C12 (mouse myoblast) was generated by cyclic electric pulses (amplitude < 60 V, 1 ms width) through electrodes of platinum wire dipped in the medium. The spectrum of fluctuating intensity of the transmitted laser beam through the myotubes, which periodically repeated contraction and relaxation, was analyzed. The results show that the designed laser system is effective to detect frequency of cyclic contractile movement of myotubes between 0.5 Hz and 5 Hz.

Citation Download Citation

Shigehiro Hashimoto, Hiroyuki Tonami, Eiji Yamada, Shuichi Mochizuki, Jun Takase, and Mieko Ohsuga "Application of laser to measurement of cyclic contractile movement of cultured myotubes", Proc. SPIE 7179, Optics in Tissue Engineering and Regenerative Medicine III, 71790D (12 February 2009); https://doi.org/10.1117/12.805234

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