Spectral histology of breast tissue using mid-infrared spectroscopic imaging

F. Nell Pounder; Rohit Bhargava

doi:10.1117/12.810125

23 February 2009 Spectral histology of breast tissue using mid-infrared spectroscopic imaging

F. Nell Pounder, Rohit Bhargava

Proceedings Volume 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII; 718206 (2009) https://doi.org/10.1117/12.810125
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Histopathologic recognition is the gold standard in breast cancer diagnoses and is a primary determinant tool for cancer research. Unfortunately, the manual nature of histopathologic recognition leads to low throughput analysis, delays in decision-making and errors. Here, we present an automated means to accurate histologic recognition using mid-infrared molecular spectroscopy. Fourier transform infrared (FT-IR) spectroscopic imaging is combined with statistical pattern recognition and high throughput sampling to provide automated tissue segmentation into constituent cell types. The method does not need dyes or probes and dispenses with human input. Results demonstrate that the technique is capable of accurate histologic segmentation that can potentially become competitive with that attained by conventional immunohistochemical analyses.

Citation Download Citation

F. Nell Pounder and Rohit Bhargava "Spectral histology of breast tissue using mid-infrared spectroscopic imaging", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 718206 (23 February 2009); https://doi.org/10.1117/12.810125

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