Dynamic molecular imaging of living cells by using Raman scattering

Katsumasa Fujita; Keisaku Hamada; Nicholas Isaac Smith; Yasushi Inouye; Satoshi Kawata

doi:10.1117/12.809053

23 February 2009 Dynamic molecular imaging of living cells by using Raman scattering

Katsumasa Fujita, Keisaku Hamada, Nicholas Isaac Smith, Yasushi Inouye, Satoshi Kawata

Proceedings Volume 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII; 71820I (2009) https://doi.org/10.1117/12.809053
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Raman spectroscopy has been utilized to investigate properties of biomolecules due to its capability of detecting molecular vibrations that represent molecular species, structures and environmental conditions. In this research, we used Raman scattering to image dynamics of molecules in living cells. By combining the 532 nm excitation and a slitscanning detection technique, we observed dynamics of molecular distributions of cytochrome, protein beta sheets, and lipids in an unstained HeLa cell during mitosis with a frame rate of 5 minutes.

Citation Download Citation

Katsumasa Fujita, Keisaku Hamada, Nicholas Isaac Smith, Yasushi Inouye, and Satoshi Kawata "Dynamic molecular imaging of living cells by using Raman scattering", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71820I (23 February 2009); https://doi.org/10.1117/12.809053

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