Probing the mechanical properties of Drosophila embryo ephitelial cells in vivo by laser nanodissection

Matteo Rauzi; Eric P. Mottay; Thomas Lecuit; Pierre-François Lenne

doi:10.1117/12.808171

12 February 2009 Probing the mechanical properties of Drosophila embryo ephitelial cells in vivo by laser nanodissection

Matteo Rauzi, Eric P. Mottay, Thomas Lecuit, Pierre-François Lenne

Author Affiliations +

Proceedings Volume 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII; 71820Y (2009) https://doi.org/10.1117/12.808171
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We used laser nanodissection to study the nature, magnitude and distribution of forces at cell-cell contacts during tissue morphogenesis in live Drosophila embryos. We designed two set-ups coupling a near infrared femtosecond laser to either an inverted fluorescence microscope or a spinning-disk confocal microscope. We show that the developed tools are able to locally disrupt the acto-myosin network while preserving the integrity of the membranes. With these systems, we could explore the redistribution of cortical elements and relaxation of cell-cell contacts after local ablation.

Citation Download Citation

Matteo Rauzi, Eric P. Mottay, Thomas Lecuit, and Pierre-François Lenne "Probing the mechanical properties of Drosophila embryo ephitelial cells in vivo by laser nanodissection", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71820Y (12 February 2009); https://doi.org/10.1117/12.808171

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