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12 February 2009 Probing the mechanical properties of Drosophila embryo ephitelial cells in vivo by laser nanodissection
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Abstract
We used laser nanodissection to study the nature, magnitude and distribution of forces at cell-cell contacts during tissue morphogenesis in live Drosophila embryos. We designed two set-ups coupling a near infrared femtosecond laser to either an inverted fluorescence microscope or a spinning-disk confocal microscope. We show that the developed tools are able to locally disrupt the acto-myosin network while preserving the integrity of the membranes. With these systems, we could explore the redistribution of cortical elements and relaxation of cell-cell contacts after local ablation.
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Matteo Rauzi, Eric P. Mottay, Thomas Lecuit, and Pierre-François Lenne "Probing the mechanical properties of Drosophila embryo ephitelial cells in vivo by laser nanodissection", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71820Y (12 February 2009); https://doi.org/10.1117/12.808171
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